LACTOSE SULFITE BROTH BASE (Eur. Pharma.) 500 grams/bottle

Lactose Sulfite Broth Base is a selective medium recommended by ISO 7937 for the confirmation of Clostridium perfringens based on lactose fermentation and production of hydrogen sulfide. The nutrient base provides optimal conditions for the development of Clostridium. Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is the source of vitamins, particularly of the B-group. Lactose is a complex carbohydrate energy source. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Cysteine hydrochloride is the reducing agent. Colonies producing hydrogen sulfide are characterized by a blackening due to the reaction of Sodium bisulfite and the Ferric ammonium citrate salt. The containers showing a blackening and abundant formation of gas in the Durham tube (at least 1/10 of the volume) indicate the presence of C. perfringens.