Sabouraud Dextrose Agar 2% is recommended for the cultivation of dermatophytes, particularly those associated with skin infections. The fungi colonies can be observed macro and microscopically. To prepare a selective culture medium, aseptically add cycloheximide, penicillin and streptomycin, since is demonstrated that the basic agar, fortified by three antibiotics, considerably improves the isolation of pathogenic fungi from heavily contaminated sources. Dextrose is the fermentable carbohydrate providing carbon and energy. Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Bacteriological agar is the solidifying agent. The high dextrose concentration makes this medium selective for fungi. The low pH of 5,6 approximately is appropriate for the growth of dermatophytes, as well as being slightly inhibitory to contaminating bacteria in clinical specimens.
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