Peptone Water (Tryptone Water) is recommended for the detection of Enterobacteriaceae, in particularly of E. coli, in water and food samples based on indole production. Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth and Sodium chloride supplies essential electrolytes for transport and osmotic balance. This medium is a good substrate for the production of indole because of its high content of tryptophan. The ability of certain organisms to break down the aminoacid tryptophan with indole formation is an important property which is used for the classification and identification of bacteria.
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