Brain Heart Infusion Broth (BHI) is a liquid medium rich in nutrients, suitable for the cultivation of several fastidious strains of bacteria, such as streptococci, meningococci and pneumococci, fungi and yeasts. BHI Broth is recommended in Standard Methods for water testing and in antimicrobial susceptibility tests. Tubes of 0,5 ml BHI Broth are used to cultivate bacteria used in the preparation of inocula for use in microdilution minimal inhibitory concentration (MIC) and identification (ID) test panels. The nutritionally rich base of beef heart and calf brain infusions and peptone mixture provide nitrogen, vitamins, minerals and amino acids essential for growth of a variety of microorganisms. Dextrose is the carbon energy source and Sodium chloride maintains the osmotic balance.
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