Acetamide Broth contains acetamide which, as a sole source of carbon in the medium, is used for the confirmation and identification of Pseudomonas aeruginosa. It uses the ability of non fermenting Gram negative bacteria to deaminate the acetamide, the deamination of the acetamide produces ammonia which increases the pH of the medium, the resulting alkalinization is shown by a color change of the Phenol red from orange-red to purple-red. Acetamide is the single carbon source. Potassium salts have a high buffering capacity. Sodium chloride supplies essential electrolytes for transport and osmotic balance and Phenol red is a pH indicator. Pseudomonas aeruginosa is an opportunist pathogen for humans which is capable of growing in water with a low concentration of nutrients. This is why natural mineral water and spring water are Pseudomonas aeruginosa free at the time of their commercialization. This microorganism can also be found in swimming pool water.
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