ASPARAGINE BROTH 500 grams/bottle

Asparagine Broth is an excellent enrichment broth for Pseudomonas aeruginosa. This medium is recommended for its enumeration and identification based on the hydrolyzation of asparagine to aspartic acid performed by the bacteria. Pseudomonas aeruginosa is an opportunist pathogen for humans, capable of growing in water with a low concentration of nutrients. This is why natural mineral water and spring water are Pseudomonas aeruginosa free at the time of their commercialization. This microorganism can also be found in swimming pool water. The formula contains a strictly mineral base with asparagine as the sole source of nitrogen, and glycerol as the carbon source. Potassium salts act as a buffer system and magnesium sulfate provides magnesium ions required in a large variety of enzymatic reactions, including DNA replication and also acts as a buffer. Since P. aeruginosa hydrolyze asparagine to aspartic acid, the appearance of growth with or without fluorescent pigmentation is considered a presumptive test for its presence. Counts are determined using the MPN tubes. Confirmation is made by subculturing a loopful from each turbid tube into Acetamide Broth (Cat. 1211).