Tryptone Bile Salts Agar is used in the quick test detection and enumeration of Escherichia coli and others coliforms in foods and waters by the membrane filtration technique. Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Bile salts are inhibitors to Gram-positive organisms and suppress coliform bacteria. Bacteriological agar is the solidifying agent. The fecal presence and level is an important factor in the evaluation of a water mass and the infection risk which it poses to human health. The analysis of water samples to detect E.coli, which usually is found in the intestinal tract of humans and other warm-blooded animals, provides an indication of this type of contamination. This medium is recommended for the quick test method which is based on the fact that 99% of Escherichia strains produce indole from tryptophan at 44°C when grown by the membrane filtration technique.
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