Welcome to the Refreshed Chemopharm Webstore!
 

COLUMBIA AGAR BASE (Eur. Pharma.) 500 grams/bottle

SKU
CDL/1104
Brand
CONDALAB
Pre-order (Deliver in 8 to 12 weeks)
Login For Price
Columbia Agar Base is a highly nutritive general purpose medium for the cultivation of fastidious organisms, especially when used as a base for Blood Chocolate Agar. It can also be used as a selective isolation medium by adding antimicrobial agents. It is suitable for the isolation and cultivation of a wide variety of microorganisms with difficult growth characteristics. By adding blood, it can be used to determine hemolytic reactions. The majority of the common pathogenic bacteria, however, grow well without the addition of blood. Pancreatic digest of casein, meat peptic digest and Heart pancreatic digest provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract and maize starch are a source of vitamins, particularly of the B-group essential for bacterial growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent. The blood is another source providing growth factors for the microorganisms and is the basis for determining hemolytic reactions. Hemolytic patterns may vary according to the type of blood or base medium used. For example, defibrinated sheep blood gives best results for Group A streptococci. To make the medium more selective, the following supplements may be added if desired: CNA Staph/Streph Supplement (Cat. 6016) for staphylococci, streptococci and pneumococci isolation. Brucella Supplement (Cat. 6017) for Brucella isolation. The European Pharmacopoeia, USP, recommends the Agar Columbia in the Paragraph 2.6.13 Microbiological examination of non-Sterile products: test for specified microorganisms for the testing of Clostridia in products. ISO 10272 indicates that Columbia Agar Base with blood can be used to allow the development of well-isolated colonies, to use these colonies in confirmation tests. With the addition of 5-10% sterile defibrinated blood and, especially with Polyenrichment Supplement (Cat. 6011) and VCN Supplement (Cat. 6013), Columbia Agar Base becomes an excellent chocolate agar that can be used to isolate pathogenic Neisseria, gonococci and meningococci, as good as or better than Thayer-Martin Medium. Depending on the selection of inhibitors, alternative supplements to VCN may be VCNT (Cat. 6026) or VCAT (Cat. 6014).
More Information
Brand CONDALAB
Write Your Own Review
You're reviewing:COLUMBIA AGAR BASE (Eur. Pharma.) 500 grams/bottle
Your Rating
More from CONDALAB
Who Viewed This Also Viewed
  1. Cole-Parmer H2200-H-E Mini Digital Incubator, Heat Only, 230 V
    Cole-Parmer H2200-H-E Mini Digital Incubator, Heat Only, 230 V Brand: COLE-PARMER SKU: CP/P-03608-03
    Login For Price
  2. Centrifuge Tubes, 15ml, Flat-top cap, Sterile, rack pack, 50 Tubes/rack, 500 Tubes/case
    Centrifuge Tubes, 15ml, Flat-top cap, Sterile, rack pack, 50 Tubes/rack, 500 Tubes/case Brand: BIOLOGIX SKU: BX/CT/ST/10-9151
    Login For Price
  3. Bottle NM HDPE 60ml 12/pk
    Bottle NM HDPE 60ml 12/pk Brand: COLE-PARMER SKU: CP/P-06035-11
    Login For Price
  4. HITRAP Q HP 5X5 ML
    HITRAP Q HP 5X5 ML Brand: CYTIVA SKU: GE/17115401
    Login For Price
  5. ECON 3-Hole Cap System 5L HPLC
    ECON 3-Hole Cap System 5L HPLC Brand: COLE-PARMER SKU: CP/P-02017-55
    Login For Price