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Wilkins Chalgren Medium was designed for use in the determination of minimum inhibitory concentrations (MIC) of antibiotics for anaerobic bacteria by the agar dilution method. It is also recommended for the isolation of anaerobic organisms from clinical specimens. It has the same performance in Petri dishes as in tubes. It has the advantage over other media in that it does not need the addition of blood to obtain the satisfactory growth of clinically important anaerobic bacteria. Tryptone and Bacteriological peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group and other growing factors to cultivate Bacteroides melaninogenicus and Peptostreptococcus anaerobius. Dextrose is the fermentable carbohydrate providing carbon and energy. L-Arginine provides amino acids for the growth of Eubacterium lentum. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium pyruvate acts as an energy source for asaccharolytic cocci such as Veillonella and to catalyze and degrade traces of hydrogen peroxide which affects the metabolism of anaerobes. Haemin and vitamin K1 are growth factors. Haemin is essential for the growth of Bacteroides species. Bacteriological agar is the solidifying agent.
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