SLANETZ-BARTLEY MEDIUM (ISO 7899-2) 500 grams/bottle

Slanetz-Bartley Medium is very selective for enterococci. Burkwall and Hartman demonstrated that the addition of 0,5 ml of Tween 80 and 20 ml of a 10% Sodium carbonate or bicarbonate solution to each liter of the medium was valuable when investigating enterococci in frozen foods. Tryptose provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Glucose is the fermentable carbohydrate providing carbon and energy. Dipotassium phosphate is a buffer. Sodium azide inhibits Gram-negative bacteria. Triphenyltetrazolium chloride is reduced to formazan by the enterococci. Bacteriological agar is the solidifying agent. It is prepared according to ISO 7899-2. This medium also complies with the recommendations of the British Ministry of Health Report 71, and the German DIN Regulations 10181 and 10160 for the examination of milk, meat and meat products.