Rogosa SL Agar is used for the isolation, enumeration and identification of lactobacilli in oral bacteriology, feces, vaginal specimens and foodstuffs. This selective medium, modified by Rogosa to contain high levels of sodium acetate and ammonium citrate at a low pH which inhibits most microorganisms, including streptococci and molds and limits swarming but allows the growth of lactobacilli. Sucrose, arabinose and dextrose are fermentable carbohydrates as carbon and energy sources. Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Sulfate salts provide inorganic ions. Polysorbate 80 is a surfactant and is incorporated to neutralize phenols, hexachlorophene and formalin. Monopotassium phosphate acts as a buffer system. Bacteriological agar is the solidifying agent.
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