MOSSEL EE BROTH (Eur. Pharma.) 500 grams/bottle

Mossel EE (Enterobacteriaceae Enrichment) Broth is as an enrichment broth, used to promote the growth of the Enterobacteriaceae group, microorganisms which contaminate foods. The European Pharmacopoeia, USP in paragraph 2.6.13: Microbiological examination of non-sterile products: test for specified microorganisms recommends this medium for the testing of bile-tolerant Gram-negative bacteria in products. The enumeration of Enterobacteriaceae is of great importance when controlling the sanitary condition of food. Microorganisms can be injured in food processing, including exposure to low temperatures, sub marginal heat, drying, radiation, preservatives or sanitizers. Recovery depends on the adequate resuscitation of damaged cells. Escherichia coli, even though present in small numbers as a contaminant in foods, grows easily in this medium. Pancreatic digest of gelatin provides nitrogen, vitamins, minerals and amino acids essential for growth. Glucose is the fermentable carbohydrate providing carbon and energy. Disodium phosphate and monopotassium phosphate act as a buffer system. Brilliant green and ox bile are selective agents, inhibiting Gram-positive microorganisms.