Rappaport Vassiliadis Broth is recommended as the selective enrichment medium when isolating Salmonella species from food and environmental specimens and is recommended by the European Pharmacopoeia in Paragraph 2.6.13 Microbiological examination of non-sterile products: test for specified microorganisms for the test of Salmonella in products. Soy peptone provides nitrogen, vitamins and amino acids, essential nutrients for growth. Potassium phosphates balance the low pH of the medium, combined with the presence of magnesium chloride to raise the osmotic pressure, and malachite green to inhibit other organisms. This medium has been found to be superior to other Salmonella selective enrichment media, especially when small inocula and a preenrichment broth are used.
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