m-EI Chromogenic Agar Base is recommended for the detection and enumeration of enterococci in water by the membrane filter technique. The medium was developed as a single-step procedure that does not require the transfer of the membrane filter to another substrate. Observation of blue color colonies confirms the presence of enterococci. A wide range of sample volumes or dilutions can be tested by this single-step membrane filtration procedure for the detection and enumeration of enterococci in potable, fresh, estuarine, marine and shellfish-growing waters. Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract provides trace elements, vitamins and amino acids. Esculin is hydrolyzed by enterococci to form esculetin and dextrose. Cycloheximide inhibits most fungi, and the sodium azide inhibits Gram negative bacteria. X-Glucoside is the substrate of the glucosidase-positive enterococci and the agar is added into the medium as a solidifying agent.
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