Sabouraud Dextrose Agar with Chloramphenicol and Cycloheximide can be used for cultivating yeasts and pathogenic fungi, particularly those associated with skin infections, and aciduric microorganisms. This medium is a modification of the Dextrose Agar described by Sabouraud, with the addition of Chloramphenicol and Cycloheximide. Chloramphenicol is an antibiotic which aids in isolating pathogenic fungi from heavily contaminated material, as it inhibits most contaminating bacteria. It is a recommended antibiotic for use with media due to its heat stability and wide bacterial spectrum. Cycloheximide is an antibiotic which inhibits saprophytic fungi but allows for the growth of pathogenic fungi: Cryptococcus neoformans, Aspergillus fumigatus and some species of Candida (albicans, krusei). Dextrose is the fermentable carbohydrate providing carbon and energy. Peptone mixture provides nitrogen, vitamins, minerals and amino acids essential for growth. Bacteriological agar is the solidifying agent. The high dextrose concentration and acidic pH make this medium selective for fungi.
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