VIOLET RED BILE AGAR WITH LACTOSE +MUG, 500g/bt 500 grams/bottle

Violet Red Bole with Lactose Agar + MUG is a selective medium used for the detection of coliforms and the fluorogenic detection of Escherichia coli. Violet Red Bile Agar with MUG is specified in many procedures to enumerate coliforms in food and dairy products. Incorporating MUG into Violet Red Bile Agar permits the detection of E. coli among the coliform colonies. Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group.Lactose is the fermentable carbohydrate providing carbon and energy. Bile salts and Crystal violet inhibit gram-positive bacteria. Neutral red is a pH indicator. Sodium chloride supplies essential electrolytes for transport and osmotic balance. The enzyme which cleaves MUG is highly specific to E. coli, making the simultaneous detection of total coliforms and E. coli possible. Bacteriological agar is the solidifying agent. It is convenient to use the pour plate method by placing 1 ml of the desired dilution in a sterile Petri dish, adding 15 ml of the medium, cooled to 45-50°C, and rotating gently before allowing solidifying. Once solidified, pour a second layer of the medium to a depth of 5 mm. Allow to solidify. Incubate at a temperature of 35±2 °C for 18-24 hours. Check the plates under UV light (366 nm). Light blue fluorescence indicates the presence of E. coli. Lactose fermenters form red colonies with red-purple halos. Occasionally cocci of the intestinal tract can develop as small, punctiform red colonies.