Tryptose Phosphate Broth II is recommended for the cultivation of fastidious and pathogenic microorganisms as well as antibiotic resistance and sentivity tests by the serial tube dilution technique. If desired, the addition of 0,1 to 0,2% agar to the broth enhances the growing of anaerobic fastidious microorganisms by delaying the dispersion and diffusion of CO2 . The low concentration of agar provides ideal condition for the aerobic growing in the upper part of the tubes and anaerobic and micro-aerobic conditions growing in the lower part of the tube. Peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. The buffering capacity is provided by the disodium phosphate.
Write Your Own Review