TRYPTOPHAN CULTURE BROTH (ISO 9308-1) 500 grams/bottle

Tryptophan Culture Broth ISO is used for the quick and standard test for the detection of E. coli and other coliforms by indole production. Indole formation depends exclusively on the development of bacteria producing enzymes called tryptophanases, which oxidize the L-tryptophan essential amino acid producing indole, skatole (methyl indole) and indole acetate. Several bacteria genera have species that are indole positive, particularly Proteus, Escherichia, Edwardsiella, Flavobacterium, Aeromonas, Plesiomonas, Bacillus, etc. Tryptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. No fermentable carbohydrates in the medium allow for the good synthesis of trypthophanase and therefore indole production.