Nitrate Broth, combinated with the appropriate reagents, is used to confirm the utilization of nitrate. Nitrate reduction has been extensively studied in bacteria due to its significance in the global nitrogen cycle. Denitrification removes nitrate, an accessible nitrogen source for plants, from the soil and converts it to N2 a much less tractable source of nitrogen that most plants cannot use. Some bacteria, as Enterobacteriaceae group, are capable of using nitrate as their terminal electron accepter. Nitrite, the product of nitrate reduction, is still a highly oxidized molecule and can be fully reduced to nitrogen gas. There are microorganisms that are able to reduce nitrate to nitrogen gas.
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