EC with MUG Fluorogenic Broth is a medium recommended for the detection of E. coli using the membrane filtration technique. Faecal contamination of water is a serious problem due to the possibility of contracting diseases from pathogens (disease-causing organisms). This medium allows a better detection of coliform organisms, in particular of E. coli, and is used to investigate drinking water, wastewater treatment systems and generally for water-quality monitoring, as well as shellfish and other foods. The medium can be incubated at 35Â±2 ºC for the detection of coliform organisms or at 44,5 ºC for the isolation of E. coli. The bile salts act as selective agent inhibiting Gram-positive bacteria, bacilli and enterococci but allowing E. coli to develop. The potassium salts have a high buffering capacity. Tryptose provides the nutrients for growth and lactose is the fermentable carbohydrate as carbon and energy source. Sodium chloride maintains the osmotic balance. E. coli contauns the enzyme ÃŸ-D-glucuronidase that hydrolyzes MUG to yield a fluorogenic product that is detectable under long-wave (366 nm) UV light. The addition of MUG to EC Broth provides another criterion, in addition to growth response and gas production, to determine the presence of E. coli in food and environmental samples.
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