Kupferberg Trichomonas Broth is used for the isolation and cultivation of Trichomonas species, and was originally formulated by Kupferberg. Trichomonas belongs to flagellate group of protozoa, organisms that parasitize the intestine and urogenital systems of humans. Trichomonas hominis is a non-pathogenic protozoan whereas Trichomonas vaginalis is a frequent cause of vaginitis. Despite wet mount examination of infected material is as efficient as cultures in revealing infections, current evidence suggests that cultivation methods are superior, which was earlier stated by Williams and Kean and Day. Kupferberg demonstrated the greater accuracy of the culture method, in addition, to observe that the efficiency of therapy for these infections could be ascertained by using negative cultures. The culture media can be made selective for the growth of Trichomonas by the external addition of antibiotics which make the media inhibitory to the accompanying bacterial flora. The Kupferberg Trichomonas Broth medium contains peptones, which provide the nitrogenous substances required for growth. Maltose acts as energy source. The selective agent chloramphenicol is inhibitory to accompanying Gram-positive and Gram-negative bacteria but not to Trichomonas species.
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