TSA Agar Nº2 Modified has been especially formulated for the production of blood plates for the isolation and cultivation of fastidious microorganisms from clinical samples, and produces enhanced hemolysis due to the special growth factors included in the formula. It is a medium which is very rich in nutrients and is recommended for general use in microbiological laboratories. It supports the abundant growth of fastidious organisms such as pneumococci, streptococci, Neisseria, etc. Containing two peptones as rich nitrogen sources, this medium supports the growth of a great variety of micro-organisms, including fastidious aerobes and anaerobes. Sodium chloride maintains the osmotic balance and the bacteriological agar is the solidifying agent. A list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacteria, Listeria, Pasteurella, Vibrio, Haemophilus vaginalis, Candida, etc. Since it lacks carbohydrates, it is very useful in the study of hemolytic reactions and also in the preparation of chocolate agar. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. Pneumococci frequently appear as very flat, smooth, translucent, grayish and sometimes mucoid colonies surrounded by a narrow zone of green (alpha) hemolysis. Hemolytic streptococci can be translucent or opaque, grayish, small (1 mm), or large matt and mucoid (2-4 mm) colonies, surrounded by a hemolysis zone. Staphylococci are seen as opaque, white to gold-yellow colonies with or without zones of beta hemolysis. Listeria produces small zones of beta hemolysis. They can be distinguished by their rod shape in stains, and by motility at room temperature.
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