Inositol Brilliant Green Bile Agar (Plesiomonas Differential Agar) is a medium described by Schubert and is recommended for the selective isolation of Plesiomonas shigelloides and Aeromonas from faeces and food samples. Plesiomonas shigelloides was originally isolated in 1947 by Ferguson and Henderson and is the only species in the genus. Humans are infected with P. shigelloides through ingestion of contaminated food or water, or by contact with colonized animals. P. shigelloides is an uncommon cause of acute bacterial gastroenteritis and an extremely rare cause of extraintestinal infections, e.g. sepsis, meningitis, cellulites, septic arthritis, and osteomyelitis. Other organisms implicated in human waterborne diarrhea include Aeromonas species. Proteose peptone and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bile salts and brilliant green inhibit gram-positive bacteria and most gram-negative bacteria except coliforms. P. shigelloides grow in the presence of these substances. Meso-inositol is a fermentable carbohydrate source. Most bacterial species do not ferment meso-inositol, but almost all strains of P. shigelloides ferment this to naturally occurring cyclic polyhydroxyl alcohol. Neutral red is the pH indicator. Bacteriological agar is the solidifying agent.
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