Luria Agar with Kanamycin 50 µg/ml (Miller's LB Agar) is used for the selective growth of Kanamycin resistant E. coli recombinant strains in molecular genetic studies. This medium is recommended for strains that require less salt concentration. The transformed E. coli are plated directly onto selective agar media (LB Agar containing antibiotic), where fewer transformed colonies will appear per ml plated. To select the bacteria with the plasmid, it is necesary to subcultivate an inoculum from LB Agar to LB Broth with the antibiotic added.
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