SCHAEDLER AGAR 500 grams/bottle

Schaedler Agar is prepared according to the formulation described by Schaedler, Dubos and Costello, and modified by Mata et al. It can easily support the growth of fastidious anaerobes from the intestinal and digestive tracts and other organs without the interference of the accompanying aerobic flora, because of its superior nutritive properties and its low oxidation-reduction potential. In normal conditions, the multiplication of anaerobes is diminished by the rapid increase of enterococci, Escherichia coli, Enterobacter and other intestinal facultative bacteria. Schaedler can be used with selective substances for the isolation and recovery of lactobacilli, streptococci, clostridia, Bacteroides, and Flavobacterium from feces and contents of the intestinal tract. Although thioglycollate is widely used to lower the oxidation-reduction potential favoring the development of anaerobes, it has been proved that it is an inhibitor of other organisms. In this case the medium includes cystine which, along with dextrose, acts as a reducing agent. Trypticasein soy broth, peptone and yeast extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate that provides carbon and energy. Tris (Hydroxymethyl Aminomethane) acts as a buffer system. Hemin stimulates organism growth. L-Cystine is a reducing agent. Bacteriological agar is the solidifying agent.