MUELLER HINTON AGAR II 500 grams/bottle

Mueller Hinton Agar II was originally developed for the cultivation and isolation of pathogenic strains of Neisseria. It was found that this medium was useful in identifying sulfonimide-resistant and responsive strains of gonococci. However, Mueller Hinton Agar is now used in antimicrobial disk susceptibility testing. Bauer and Kirby developed a standardized procedure for determining the susceptibility of bacteria to antibiotic, in which Mueller Hinton Agar was selected as the test medium, because clinical microbiology laboratories in the 60s were using a wide variety of different mediums and procedures. Its performance is specified by CLSI (Clinical and Laboratory Standards Institute), and it is recommended for testing rapidly growing aerobic or facultatively anaerobic bacterial pathogens and fastidious species like H. influenziae, N. gonorrhoeae or S. pneumoniae, when desfibrinated sheep blood is added. Mueller Hinton Agar II is manufactured to contain low levels of thymine and thymidine, and controlled levels ofcalcium and magnesium. The use of a medium with suitable growth characteristics is essential to test susceptibility of microorganisms to antibiotics. It is also recommended for testing most commonly encountered aerobic and facultative anaerobic bacteria. Beef infusion and acid Casein peptone (H) provide nitrogen, vitamins, minerals and amino acids essential for growth. The starch absorbs any toxic metabolites produced. Bacteriological agar is the solidifying agent.