COLUMN PD 10

Details

PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.

Flexible run protocolsÂ â€“ perform desalting and buffer exchange using gravity flow (1.0 to 2.5 mL samples) or centrifugation (1.75 to 2.5 mL samples)
Flexible throughputÂ â€“ desalt one sample or multiple samples in parallel
Good recoveryÂ â€“ in the range of 70% to > 95%
High desalting capacityÂ â€“ remove > 98% salt with gravity; > 90% with centrifugation
Minimal dilutionÂ â€“ typically none when used as spin columns; 1.4Ã— when used with gravity flow
Time savings compared to dialysisÂ â€“ inexpensive and convenient alternative to lengthy and troublesome dialysis procedures

What comes in the PD-10 column package

Each product pack includes 30 columns, a buffer tray, a column stand, and 4 adapters for centrifugation.

Versatile for use with centrifugation or gravity flow

The product instructions include two protocols. The gravity protocol allows simple processing of samples using one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.

Successful use of these prepacked desalting columns

The following publications demonstrate the utility of PD-10 columns:

desalting of membrane proteinsÂ after anion exchange chromatography
desalting of variantsÂ of an essential reductase involved in fatty acid biosynthesis
buffer exchange of an affinity-taggedÂ acyl carrier protein

Accessories for PD-10 Desalting Columns

AÂ LabMate buffer reservoirÂ can be used for one-step application of the column wash and equilibration buffer using the gravity flow protocol. It is not included with this product but can be purchased separately.

Other Sephadex G-25 columns from Cytiva

Cytiva's solutions for desalting include a wide range of columns containing Sephadex G-25 resin. Options are available to accommodate sample volumes from 100 μL to 15 mL and use with manual methods or a chromatography system. Let Cytiva help youÂ find the right desalting product.

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