Welcome to the Refreshed Chemopharm Webstore!
 

PRESCISSION PROTEASE (STORE IN FREEZER : -20 DEG C)

SKU
GE/27084301
Brand
CYTIVA
Pre-order (Deliver in 8 to 12 weeks)
Login For Price

PreScission Protease is a fusion protein of human rhinovirus (HRV) 3C protease and GST. It allows for on-column cleavage of GST tags and protein purification in one step.

  • Specific cleavage – between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.
  • Time-saving – GST-tagged proteins can be cleaved while still bound to an affinity resin.
  • Low incubation temperature – enables low temperature cleavage of fusion proteins containing the HRV 3C protease recognition sequence.

On-column or off-column enzyme cleavage

GST tags and protease can be removed in a single step while fusion proteins are still bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow affinity resin. Another option is to elute the GST-tagged protein using reduced glutathione, cleave, then pass the sample over the same resin to remove the protease and tags.
Unlike other common proteases, such as thrombin and Factor Xa, this GST-tagged fusion protein can be removed easily through binding to a resin with affinity for GST. Other non-recombinant proteases require an additional purification step with a different resin.

Vectors encoding the HRV 3C protease recognition sequence

pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 encode the recognition sequence for this 3C protease.

Successful use in published research

PreScission Protease is used for enzyme cleavage in a variety of research applications, including:
More Information
Brand CYTIVA
Write Your Own Review
You're reviewing:PRESCISSION PROTEASE (STORE IN FREEZER : -20 DEG C)
Your Rating
More from CYTIVA
Who Viewed This Also Viewed
  1. BD-S 115- 230V, Solid.Line incubators with natural convection, 118L, 50Hz
    BD-S 115- 230V, Solid.Line incubators with natural convection, 118L, 50Hz Brand: BINDER SKU: BN/9090-0022
    Login For Price
  2. Shaker F/8in Sieves 220V 50Hz
    Shaker F/8in Sieves 220V 50Hz Brand: COLE-PARMER SKU: CP/OO/O/P-59986-62
    Login For Price
  3. General Purpose Dish 44mm 100/pk
    General Purpose Dish 44mm 100/pk Brand: COLE-PARMER SKU: CP/P-01018-60
    Login For Price
  4. Tube Micro 2.0ml PP Str 100/pk
    Tube Micro 2.0ml PP Str 100/pk Brand: COLE-PARMER SKU: CP/P-02560-38
    Login For Price
  5. Beaker Tri-Corner 800ml 100/pk
    Beaker Tri-Corner 800ml 100/pk Brand: COLE-PARMER SKU: CP/P-06020-09
    Login For Price