Welcome to the Refreshed Chemopharm Webstore!
 

NI SEPHAROSE HP 25 ML

SKU
GE/17526801
Brand
CYTIVA (LIFESCIENCE)
Pre-order (Deliver in 8 to 12 weeks)
Login For Price

Ni Sepharose High Performance nickel-charged IMAC resin is for high-resolution his-tagged protein purification with high binding capacity for maximized recovery.

  • High resolution – due to small bead size (average bead size is 34 µm).
  • High binding capacity for high protein recovery – exceeds 40 mg histidine-tagged (his-tagged) protein/mL resin.
  • Broad reagent compatibility – compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Nickel resin for high recovery of histidine-tagged proteins

Ni Sepharose High Performance (HP) affinity resin consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with nickel, which selectively retains proteins with exposed histidine groups.
This IMAC resin has a binding capacity that exceeds 40 mg/mL, resulting in high protein recovery. The small bead size contributes to a more efficient purification compared to nickel resins of larger bead size.

Successful use in published research

Applications of this affinity chromatography resin include:

Compatible empty columns

Cytiva offers a variety of empty columns for packing your own columns. Ni Sepharose High Performance is compatible for use with empty HiScale, Tricorn, and XK columns.

Convenient prepacked columns

Ni Sepharose High Performance resin is available in the following prepacked formats:
  • HisTrap HP column: HiTrap format (1 mL or 5 mL) for convenient use with a syringe, a peristaltic pump, or a chromatography system.
  • His SpinTrap: Spin columns for screening of small sample quantities using a benchtop centrifuge.
  • His MultiTrap HP: 96-well plate for high throughput screening and small scale purification using centrifuge or vacuum equipment.
  • His Mag Sepharose Ni magnetic beads: designed for small-scale purification and screening.

Other IMAC affinity chromatography resins and columns

Cytiva's solutions include a variety of nickel-charged resins and other IMAC products. Options for high purity, high resolution, direct loading of cell culture supernatants, loading of unclarified E. coli lysates, and charging with your metal ion of choice are available.
More Information
Brand CYTIVA (LIFESCIENCE)
Write Your Own Review
You're reviewing:NI SEPHAROSE HP 25 ML
Your Rating
Who Viewed This Also Viewed
  1. OE67 Membrane Circles, Cellulose acetate, 0.45um, 85mm 50/pk
    OE67 Membrane Circles, Cellulose acetate, 0.45um, 85mm 50/pk Brand: WHATMAN SKU: WS/MI/A/10404044
    Login For Price
  2. Sterile Filters,045um White Grid,47mm/100
    Sterile Filters,045um White Grid,47mm/100 Brand: WHATMAN SKU: WH/MI/AO/7141114
    Login For Price
  3. SP SEPHAROSE FAST FLOW,  25 ML
    SP SEPHAROSE FAST FLOW, 25 ML Brand: CYTIVA (LIFESCIENCE) SKU: GE/17072910
    Login For Price
  4. Beaker Polypropylene 1000ml 3/pk
    Beaker Polypropylene 1000ml 3/pk Brand: COLE-PARMER SKU: CP/OO/O/P-06003-71
    Login For Price
  5. Bottles Square HDPE 2000ml 6/pk
    Bottles Square HDPE 2000ml 6/pk Brand: COLE-PARMER SKU: CP/OO/O/P-06019-82
    Login For Price