HIPREP 16/60 SEPHACRYL S-200 HR

Details

HiPrep Sephacryl S-200 HR (high-resolution) size exclusion chromatography columns are designed for routine protein purification at laboratory scale.

Broad fractionation range: M_rÂ ^~Â 5 000 toÂ ^~Â 250 000 (globular proteins), M_rÂ ^~Â 1 000 toÂ ^~Â 80 000 (dextrans) suitable for varied applications, including purification of recombinant tagged proteins.
High capacity columns: Preparative separation of protein in sample volumes up to 5 mL or 13 mL for 16/60 and 26/60 columns, respectively.
Convenient: Pre-packed Sephacryl columns are easily connected to Ã„KTA systems and other chromatography systems for automated chromatography at low to medium pressure.

Fast and reproducible, for routine protein purification

Sephacryl resins are dextran-acrylamide copolymers with extensive cross-linking, which gives good rigidity and chemical stability. The high resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl resins an excellent option for routine purification by size exclusion chromatography (SEC). SEC is also known as gel filtration chromatography.

Broad fractionation range (5 â€“ 250 kDa) for diverse applications

Sephacryl S-200 HR resin has been used for decades to separate biomolecules across a wide molecular weight range. Examples include:

isolation and separation of correctly dimerizedÂ recombinant tagged proteinsÂ from monomer subunits following affinity purification
study of degradation of human immunoglobulins and hemoglobin by a molecular weight 60 000 cysteine proteinase ofÂ Trichomonas vaginalis
release ofÂ monocyte caspase-1Â in a stable, active high molecular weight complex
differential phosphorylation of plant translation initiation factors byÂ Arabidopsis thaliana CK2 holoenzymes

Other Sephacryl S-200 HR formats

Sephacryl S-200 HR is also available as bulk resin for packing columns of your own.

Other Cytiva size exclusion chromatography columns

Life Sciences solutions from Cytiva include a comprehensive range of size exclusion chromatography columns and resins. For higher resolution, HiLoad Superdex 200 pg and HiLoad Superdex 75 pg columns are available. Not sure which product to use Let Cytiva help youÂ select the right size exclusion chromatography productÂ for your needs.

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