Vegetable LB Agar (Lennox) is a nutritionally rich medium based on the LB Agar (Lennox) (Cat. 1083) designed as an alternative to classical animal-based media for the growth and maintenance of pure cultures of recombinant strains of E. coli used in molecular microbiology procedures. These strains are generally derived from E. coli K12, which are unable to produce vitamin B, so this media is formulated to enhance the growth of nutritionally demanding microorganisms. This strain of E. coli has been further modified through specific mutation to create an auxotrophic strain that is not capable of growth on nutritionally deficient media. In this case, the ingredients are animal-free in order to minimize the risk of bovine spongiform encephalopathy in culture media containing bovine materials. Vegetable origin peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins, particularly the B-group essential for bacterial growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent. Vegetable LB Agar (Lennox) has a different sodium chloride level than other media such as Luria Agar (Miller LB Agar) (Cat. 1552) or Luria Agar (Miller's Modification) (Cat. 1308). This allows to select the optimum salt concentration of the medium for a specific strain.
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