Glucose Chloramphenicol Broth is recommended by the International Dairy Federation (FIL-IDF) for the isolation and enumeration of yeast and molds in milk and dairy products, using the most probable number (MPN) method. Yeast extract is the water-soluble portion of hydrolyzed yeast and is a source of vitamins, particularly of the B-group, and other growth nutrients that stimulate yeast and mold development. Glucose is the fermentable carbohydrate as a carbon and energy source. Chloramphenicol is an antibiotic which aids in isolating pathogenic fungi from heavily contaminated material, as it inhibits most contaminating bacteria. It is a recommended antibiotic for use with media due to its heat stability and wide bacterial spectrum.
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