GN Enrichment Broth (Hajna) was developed by Hajna for the selective enrichment of enteric Gram-negative microorganisms. GN stands for Gram-negative. It is intended for use in the detection of Salmonella spp. and Shigella spp. from clinical and non-clinical specimens. Tryptose provides nitrogen, vitamins, minerals and amino acids essential for growth. Mannitol and dextrose are the fermentable carbohydrates providing carbon and energy. Mannitol is provided in a higher concentration than dextrose to enhance the growth of mannitol-fermenting species, such as Salmonella and Shigella, and limits the growth of Proteus and other dextrose-fermenting bacteria. Sodium desoxycholate and sodium citrate inhibit the growth of Gram-positive organisms. Potassium phosphate is a reagent with a very high buffering capacity. Most potassium phosphate buffer solutions consist of mixtures of the monobasic and dibasic forms of potassium phosphate to varying degrees, depending on the desired pH.
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