CON-A SEPHAROSE 4B 5 ML (STORE IN CHILLER : 2 TO 8 DEG C)

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Con A Sepharose 4B with immobilized Concanavalin A is an affinity chromatography resin for capture of glycosylated biomolecules including glycoproteins and glycolipids. Stronger affinity for a-D-glucose than a-D-mannose

Concanavalin A (Con A) binds molecules that contain a-D-mannose, a-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
Group-specific adsorbent for molecules containing sugars.
Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
Concanavalin A (Con A) binds molecules that contain a-D-mannose, a-D-glucose and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
Highly suitable for isolation of cell surface glycoproteins from detergent-solubilized membranes
Con A coupled to Sepharose 4B via cyanogen bromide activation

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method. Concanavalin A (Con A) is a tetrameric metalloprotein isolated fromÂ Canavalia ensiformisÂ (jack bean). Con A binds molcules containing a-D-mannopyranosyl, a-D-glucopyranosyl and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids

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