Brain Heart Infusion Broth ISO (BHI Broth) is a general purpose medium recommended by the ISO normative 6888-1 for the confirmation of coagulase-positive staphylococci. Calf brain infusion, enzymatic digest of animal tissue and beef heart infusion provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium hydrogenphosphate is a buffer. Glucose is the fermentable carbohydrate providing carbon and energy. The coagulase test is considered positive if the clot volume is more than half of the original liquid volume.
Write Your Own Review