PPLO AGAR BASE w/o CRYSTAL VIOLET 500 grams/bottle

PPLO Agar Base w/o Crystal Violet is used in the study of the growth requirements of Mycoplasma, along with the identification and cultivation of this organism. PPLO stands for Pleuro Pneumonia-Like Organisms and was described by Morton, Smith and Leberman. Peptone and beef heart infusion provide the nutrients for growth: nitrogen, vitamins, minerals and amino acids, whilst the sodium chloride provides the osmotic balance. Crystal violet is not included in this formula since it is inhibitory on some Mycoplasma. Bacteriological agar is the solidifying agent. PPLO colonies have a round shape and a a dense center with a less dense periphery, giving a fried egg appearance on agar.