Peptone Water With Lactose is used for the confirmation of coliform presence in a water. Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Lactose is the fermentable carbohydrate providing carbon and energy. Phenol red is a pH indicator and Sodium chloride supplies essential electrolytes for transport and osmotic balance. Coliforms ferments lactose with gas and acid production. Acid formation changes the pH of medium with a resulting color change from red to yellow. When lactose is not fermented the color of medium remains red.
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