OF Basal Medium (Hugh And Leifson) is a semisolid medium, prepared according to Hugh and Leifsons formula, and is used to determine the metabolism (oxidation, fermentation) of Gram-negative bacteria. It is useful for Pseudomonas, Salmonella, Shigella and Alcaligenes. Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Bromothymol blue is the pH indicator. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Bacteriological agar is the solidifying agent.
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