Nutrient Agar Dev Regulations is a general purpose medium, not selective but suitable for the cultivation of a wide variety of microorganisms. It is recommended by the German Standard Methods (Deutsche Einheitsverfahren), the German Drinking Water Regulations (Trinkwasser-Verordnung) (1990) and the German Regulation For Food Examination (LMBG). The American Public Health Association (APHA) suggested this standard culture medium for use in bacterial processing for water analysis. In Standard Methods of Water Analysis and Standard Methods of Milk Analysis, the APHA advocated the use of dehydrated media for the bacterial examination of water and milk. Meat peptone and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent. Since this medium contains sodium chloride it can be used as a base for enrichment with blood or other supplements for cultivating fastidious microorganisms.
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