TEMPLIPHI 100AMPLIFICATION KIT (STORE IN DEEP FREEZER : -70 DEG C)

One-tube, one-temperature format

TempliPhi kits use a unique kind of circular DNA amplification to efficiently prepare micrograms of DNA from picogram circular DNA starting material. TempliPhi kits use isothermal rolling circle amplification (RCA) for the exponential amplification of circular DNA using bacteriophage Phi29 DNA polymerase. The amplification used is often called multiple strand displacement amplification, or MDA. Phi29 DNA polymerase is active at 30°C, and isothermal amplification is performed at this temperature without the need for thermal cycling. The TempliPhi protocol allows amplification of 96 samples from bacterial colonies with less than 20 min of hands-on time. Two different types of TempliPhi DNA Amplification Kits are available to meet different throughput requirements. TempliPhi DNA Amplification Kit for 2000 reactions, designed for automation, contains pre-mixed components and requires overnight incubation. TempliPhi 100 and TempliPhi 500 reaction kits contain separate, unmixed components for enhanced stability and preparations are ready in 4 to 6 hours.