DNA SILVER STAINING KIT

PlusOne DNA Silver Staining Kit kit contains all essential components for staining of nucleic acids in polyacrylamide gels. To avoid transportation of large solution volumes, most of the solutions are in a 5X concentrated form, ready to use after a simple dilution. The high sensitivity of the visualization technique allows detection of nucleic acids down to 20 to 50 pg DNA/band. The method is reliable and reproducible and gives a gel with distinct bands on an essentially colorless background, ready for drying and/or evaluation in about 1.5 h. The formulation of the kit minimizes production of toxic waste and optimizes sensitivity and robustness, making the kit especially suitable for use in automatic staining equipment: Hoefer Automated Gel Stainer and GeneStain Automated Gel Stainer. In additon, the kit also provides the method of choice for standard manual silver staining of DNA in polyacrylamide gels. The kit performs best on 0.5–1.0 mm thick plastic-backed gels, or 0.75–1.5 mm thick unbacked gels. The procedure described in this instruction is based upon the use of 125 ml solution per process step. Each kit contains material for 20 processes.