Welcome to the Refreshed Chemopharm Webstore!
 

GSTRAP FF, 5X1ML

SKU
GE/17513001
Brand
CYTIVA
Pre-order (Deliver in 8 to 12 weeks)
Login For Price

GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.

  • Fast and simple one-step purification of GST-tagged proteins.
  • Mild elution conditions preserving protein antigenicity and function.
  • Easy scale-up by connecting the columns in series.
  • Sepharose 4 Fast Flow provides good flow properties.
GST-tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap FF.GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The resin, Glutathione Sepharose 4 Fast Flow, is also available as lab packages and is an excellent choice for scale-up.The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.
More Information
Brand CYTIVA
Write Your Own Review
You're reviewing:GSTRAP FF, 5X1ML
Your Rating
More from CYTIVA
Who Viewed This Also Viewed
  1. ANTIBIOTIC MEDIUM Nº 3 500 grams/bottle
    ANTIBIOTIC MEDIUM Nº 3 500 grams/bottle Brand: CONDALAB SKU: CDL/1534
    Login For Price
  2. Sterile Filters,045um White Grid,47mm/100
    Sterile Filters,045um White Grid,47mm/100 Brand: WHATMAN SKU: WH/MI/AO/7141114
    Login For Price
  3. Heating Tape 416W 240V
    Heating Tape 416W 240V Brand: COLE-PARMER SKU: CP/OO/O/P-03106-41
    Login For Price
  4. Flowmeter,Brass/316SS
    Flowmeter,Brass/316SS Brand: MASTERFx SKU: CP/P-03295-14
    Login For Price
  5. No. 540 Filter Circles 12.5cm/100
    No. 540 Filter Circles 12.5cm/100 Brand: WHATMAN SKU: WH/MA/BO/1540125
    Login For Price