GLUCOSE OF MEDIUM ISO 21528-2:2017-08 500 grams/bottle

Glucose OF Medium is used to confirm Enterobacteriaceae colonies in products intended for human consumption and the feeding of animals, and environmental samples in the area of primary production, food production and food handling. Enzymatic digest of casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system. Glucose is the fermentable carbohydrate providing carbon and energy. Bromothymol blue is the pH indicator. Bacteriological agar is the solidifying agent. Colonies of presumptive Enterobacteriaceae should be confirmed by means of tests for the fermentation of glucose and the presence of oxidase. If the colonies are oxidase-negative and glucose-positive, the sample shall be regarded as being positive for Enterobacteriaceae. ISO 21528 recommends this medium to confirm the presumptive colonies of Enterobacteriaceae by fermentation test.