EE Broth is a medium recommended to detect and enumerate Enterobacteriaceae by the MPN method (Most probable number method). Pancreatic digest of gelatin and glucose are the nitrogen and energy sources. Dehydrated ox bile and Brilliant green inhibit gram positive bacteria and most gram negative bacteria. Sodium phosphate and potassium phosphate act as a buffer system. ISO 21528-1:2004 outlines a method, including preenrichment, for the detection of Enterobacteriaceae. It can be applied to products for human consumption and the feeding of animals, as well as environmental samples in the area of food production and food handling. This method is used when the microorganisms sought are expected to need resuscitation before enrichment, and when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.
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