DNase Test Agar with Toluidine Blue (Deoxyribonuclease Activity) is used to differentiate microorganisms with DNase activity. This differential medium is especially recommended for the identification of pathogenic Enterobacteria. Casein peptone and soy peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Deoxyribonucleic acid enables the detection of DNase that depolymerize DNA. Bacteriological agar is the solidifying agent. DNase Test Agar with Toluidine Blue contains a metachromatic dye to eliminate the necessity of reagent addition to the agar following incubation. Toluidine blue may be toxic to some gram-positive cocci and, therefore, should be used primarily with Enterobacteriaceae.
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