DEAE SEPHAROSE FAST FLOW 500 ML

The highly crosslinked agarose resin enables high flow rates and high productivity. Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, and PreDictor RoboColumns™, and HiTrap columns.

Successful use – research scale

DEAE Sepharose Fast Flow resin is used in a variety of research applications, including:

• Separation of PI 3-Kinases from guinea pig neutrophils
• Purification of myosin phosphatase from chicken smooth muscle

Compatible empty columns – research scale

Cytiva's solutions include a variety of empty columns for research scale. This DEAE resin is compatible with two of these lines, Tricorn and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications for this DEAE resin, Cytiva recommends AxiChrom columns. DEAE Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Prepacked columns – research scale

This weak anion exchange resin is available in the following formats:

• HiScreen DEAE FF for scale up and process development
• HiPrep DEAE FF columns for convenient scale-up

Prepacked columns – bioprocess scale

Prepacked ReadyToProcess columns for bioprocess applications are available on request.