Brilliant Green Tetrathionate Bile Broth was recommended by the European Pharmacopoeia for the test of Salmonella in non-sterile products as a selective enrichment broth. Meat peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Ox bile and brilliant green inhibit Gram-positive bacteria and most Gram-negative bacteria. They also prevent the growth of the anaerobic lactose fermenters such as Clostridium perfringens, which could give false positive reactions at 44 °C. The calcium carbonate is a neutralizer that will absorb any toxic metabolites. Organisms that have the enzyme tetrathionate reductase will grow and multiply in this medium due to the presence of potassium tetrathionate and Sodium chloride supplies essential electrolytes for transport and osmotic balance.
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