StrepTactin Sepharose High Performance 10ml (Store in Chiller at 2 to 8 DEG C)

StrepTactin™ Sepharose High Performance resin is designed forÂ Strep-tag II™ recombinant protein purification.

Yields high-purity tagged recombinant proteins eluted in concentrated, small volumes.
Physiological conditions and mild elution preserve target protein activity.
Fast and easy regeneration with 0.5 M NaOH.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Stable in all commonly used buffers.

StrepTactin Sepharose agarose resin

Strep-tag II is small (8 aa), which often makes tag removal unnecessary after purification. StrepTactin Sepharose High Performance has been specially developed for high specificity towards Strep-tag II to ensure purity.

The resinâ€™s small bead size (34 Âµm) allows elution in narrow peaks. Purification and elution conditions are designed to be mild and mimic physiological conditions, preserving the target proteinâ€™s activity. This robust and stable Sepharose-based resin yields reproducible purifications and minimizes the need for further concentration steps. See our StrepTactin Sepharose application note for an example of a single-step purification that achieved greater than 95% purity.

Successful use in published research

StrepTactin Sepharose is used for tagged protein purification in a variety of applications, including:

Study of the formation and structure of aÂ NAIP5-NLRC4 inflammasome
Analysis of how aÂ staphylococcal protein evades toll-like receptor activation

Compatible empty columns

Cytiva solutions include a variety of empty columns for packing your own columns. StrepTactin Sepharose is compatible for use with Tricorn and XK columns.