SP SEPHAROSE FAST FLOW, 25 ML

Composed of crosslinked 6% agarose beads with sulphopropyl (SP) strong cation exchange groups, this resin has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with these SP media (resins) is straightforward. Scale-up is further aided by a range of prepacked process development tools, for example PreDictor 96-well plates, PreDictor RoboColumns™, and HiTrap columns. For even faster flow rates and better binding capacity in scale-up, Capto S and other Capto resins are a good option.

Successful use – research scale

SP Sepharose Fast Flow media (resins) are used in a variety of research applications, including:

• Two-step ion exchange chromatography of human calprotectin from neutrophils for structural studies
• Purification of APT1 from rat liver cytosol
• Purification of a manganese peroxidase from Panaeolus sphinctrinus

Compatible empty columns – research scale

Cytiva's solutions include a variety of empty columns for research scale. SP Sepharose Fast Flow is compatible with two of these lines, Tricorn and XK columns.

Compatible empty columns – bioprocess scale

For process-scale applications, Cytiva recommends AxiChrom columns. SP Sepharose Fast Flow can also be packed in the other column platforms such as BPG and Chromaflow.

Prepacked columns – research scale

This strong cation exchange resin is available in the following prepacked formats:

• HiTrap SP FF columns – convenient for easy use with a syringe, peristaltic pump, or chromatography system such as ÄKTA system
• HiScreen SP FF columns for scale up and process development.
• HiPrep SP FF columns for convenient scale-up.

Prepacked columns – bioprocess scale

Prepacked ReadyToProcess columns are available for bioprocess applications.