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PPLO Broth Base w/o Crystal Violet is used in the studies of the growth requirements of Mycoplasma, along with the identification and cultivation of this organism. PPLO stands for Pleuro Pneumonia-Like Organisms, an old name for Mycoplasma. PPLO Broth w/o Crystal violet is prepared according to the formula described by Morton and Lecce. Peptone and beef heart infusion provide nitrogen, vitamins, minerals and amino acids essential for growth, whilst the sodium chloride provides the osmotic balance. Crystal violet is not included in this formula since it is inhibitory on some Mycoplasma. Although some species are normal human respiratory tract flora, Mycoplasma pneumoniae is a major cause of respiratory disease (primary atypical pneumonia, sometimes called walking pneumonia). Mycoplasma hominis, Mycoplasma genitalium and Ureaplasma urealyticum are important colonizers (and possible pathogens) of the human genital tract.
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